Regulatory

Part:BBa_K235033

Designed by: Natasha Tuskovich   Group: iGEM09_VictoriaBC   (2009-10-19)

[K235030][K235031] (constituitive promoter+32°C RBS thermometer+LacI+stop)

This part was designed to produce the LacI repressor above 32°C, but was not tested in time for the 2009 competition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None